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The Joker, Jack Nicholson - Tim Burton's 'Batman' (1989) is one of the most divisive takes in the entire film series, with fans either loving or hating the cartoonish portrayal. The film famously featured one of the strongest Joker performances of all time, by none other than Jack Nicholson.

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First since the 1960s - Nicholson was the first actor to portray the Joker on the big screen since Cesar Romero did it in 1966. Perhaps most importantly, Nicholson was the first to portray The Joker in the dark, maniacal way he was written in the comics.

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Warning to Heath Ledger - Heath Ledger was reportedly warned by Jack Nicholson over the role's mental and emotional toll ahead of filming. Nicholson said he could barely sleep while in character for the movie.

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Scarecrow, Cillian Murphy - The only villain to appear in every 'Dark Knight' movie, Cillian Murphy's Scarecrow tends to get overshadowed by the other seminal performances of the series.

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Arkham Asylum psychiatrist - Known as Jonathan Crane, the psychiatrist at Arkham Asylum experiments on patients starting in 'Batman Begins' (2005) through to Bane's reign of terror in 'The Dark Knight Rises' (2012).

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Poison Ivy, Uma Thurman - Uma Thurman is the only actress to portray Poison Ivy in a live-action film, though the 'Batman & Robin' (1997) performance was largely panned. The character Poison Ivy has appeared on the TV show 'Gotham,' however.

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The Penguin, Danny DeVito - Danny DeVito has never looked so horrifying as he did in 'Batman Returns' (1992). The Penguin's makeup took four and a half hours to apply.

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The Penguin, Danny DeVito - DeVito is one of two actors to play the Penguin in a live-action film, and his performance was nothing short of shocking. In the 'Gotham' TV show, of which DeVito is a fan, Robin Lord Taylor stars as the Penguin and is among the show's best actors.

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The Penguin, Burgess Meredith - The only other live-action portrayal of the Penguin in a film was Burgess Meredith in 'Batman: The Movie' (1966). Meredith also played the Penguin in the 'Batman' TV show from the same year.

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'Batman: The Movie' (1966) villains - With Burgess Meredith as the Penguin, Frank Gorshin as the Riddler, Lee Meriwether as Catwoman, and Cesar Romero as the Joker, this villainous cast of 'Batman: The Movie' was the first to define the comic book characters on the big screen.

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Dehydrating world leaders - In 'Batman: The Movie', these four super villains dehydrate important world leaders, holding them hostage until Batman and Robin meet their demands.

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Miranda Tate/Talia al Ghul, Marion Cotillard - Marion Cotillard portrays Miranda Tate, who is really Talia al Ghul, in 'The Dark Knight Rises' (2012), literally stabbing Batman in the back.

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Mr. Freeze, Arnold Schwarzenegger - Arnold Schwarzenegger is the only actor to ever portray Mr. Freeze in a live-action movie, doing so in 'Batman & Robin' (1997). TV appearances in the 1966 'Batman' and 'Gotham' proved unpopular, and the character has largely gone untouched since.

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Catwoman, Michelle Pfeiffer - Catwoman is one of the most recurring villains and has probably been played by the most actors. Michelle Pfeiffer gave an unforgettable performance in skin-tight leather in 'Batman Returns' (1992).

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Catwoman and Penguin - In 'Batman Returns,' Catwoman teams up with Danny DeVito's Penguin to frame Batman for a crime. The Catwoman character always has a dubious relationship with good and bad, often being at odds with Batman but fighting his enemies too.

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Catwoman, Halle Berry - It's pretty rare for a Batman villain to get their own movie, and the Halle Berry role created a lot of excitement and anticipation ahead of 'Catwoman' (2004).

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No one had fun - But unfortunately the movie was panned by audiences, and Berry herself said that no one on the set had fun.

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Catwoman, Anne Hathaway - Anne Hathaway played Catwoman in 'The Dark Knight Rises,' giving one of the best reprisals of the role that's been seen in the modern era.

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Two-Face, Tommy Lee Jones - Tommy Lee Jones gave one of the seminal performances of Two-Face in 'Batman Forever' (1995), a character not seen again until 'The Dark Knight' (2008).

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Dynamic - Though Tommy Lee Jones reportedly hated working with Jim Carrey, the Two-Face/Riddler dynamic was one of villainous marvel and hilarity.

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Sugar, Drew Barrymore - Drew Barrymore played Sugar, the nicer one of Two-Face's two girlfriends in 'Batman Forever' (1995).

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Spice, Debi Mazar - The meaner one was Debi Mazar's Spice in 'Batman Forever' (1995). Both Sugar and Spice operated as henchwomen for Two-Face.

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The Riddler, Jim Carrey - Originally written for Robin Williams, Jim Carrey's portrayal of the Riddler in 'Batman Forever' has divided fans. What's almost universally agreed upon is that the character is possibly the most unorthodox portrayal in any Batman movie.

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Blank sheet - Like the Penguin, the Riddler hadn't been portrayed in live action on the big screen since 1966, and he hasn't been since. However the TV series 'Gotham' has a promising Riddler character.

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Stole the show? - Carrey would later muse to The Hollywood Reporter that Tommy Lee Jones hated him for being the star and taking the movie's spotlight. Despite that, Carrey called Jones "a phenomenal actor."

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Ra's al Ghul, Liam Neeson - As the head of The League of Shadows, where Batman learns his craft, Liam Neeson plays the lead instructor Ra's al Ghul in 'Batman Begins' (2005) and 'The Dark Knight Rises' (2012).

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Character twist - While Ra's al Ghul proves a mentor for Batman at first, he later becomes a villain. Bane, who also trained in The League of Shadows, enlists with Ra's al Ghul to go against Batman.

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Ra's al Ghul's Decoy, Ken Watanabe - In 'Batman Begins', Ken Watanabe is at first believed to be Ra's al Ghul, serving as a decoy.

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